BGC0002107 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002107 (Agrobacterium fabrum str. C58)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRP-metallophore,NRPS,T1PKS	1	53,477

BGC0002107 - Region 1 - NRP-metallophore,NRPS,T1PKS

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Location: 1 - 53,477 nt. (total: 53,477 nt) Show pHMM detection rules used

Region on contig edge.

NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene overview

NRPS/PKS domains

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

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NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 53477:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (bAla) + (ohmal) + (X) + (ohmal) + (Ala - Cys) + (Gly - D-Ala)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

Atu3670

Search NORINE for peptide: strict or relaxed

AMP-binding (520..912): bAla

nrpys: bAla

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGVIWK
Nearest Stachelhaus code(s):: bAla DILQLGVIWK (100% 8Å match); Gly DILQLGVIWK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

Atu3672

PKS_AT (568..849): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 171.2
inactive: 90.3
Methoxymalonyl-CoA: 58.2
Methylmalonyl-CoA: 52.0
Propionyl-CoA: 43.8
2-Methylbutyryl-CoA: 28.3
Ethylmalonyl-CoA: 27.9
CHC-CoA: 21.4
Benzoyl-CoA: 20.8
fatty_acid: 17.9
Isobutyryl-CoA: 17.6
trans-1,2-CPDA: 17.2
Acetyl-CoA: 11.3
3-Methylbutyryl-CoA: 10.4

Atu3673

CAL_domain (173..507): pk

minowa: NH2

Prediction, score:

NH2: 101.6
Acetyl-CoA: 40.9
fatty_acid: 12.6
AHBA: 12.4
shikimic_acid: 0.0

Atu3675

AMP-binding (537..929): X

nrpys: 3-ohTyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAPTLGAVNK
Nearest Stachelhaus code(s):: 3-ohTyr DAPTLGAVNK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Atu3681

PKS_AT (537..824): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 156.9
inactive: 92.6
Methoxymalonyl-CoA: 56.4
Methylmalonyl-CoA: 53.8
Propionyl-CoA: 39.6
2-Methylbutyryl-CoA: 24.4
CHC-CoA: 21.9
Benzoyl-CoA: 21.4
Isobutyryl-CoA: 19.5
Ethylmalonyl-CoA: 15.4
3-Methylbutyryl-CoA: 14.3
trans-1,2-CPDA: 12.6
Acetyl-CoA: 11.9
fatty_acid: 8.7

Atu3682

Search NORINE for peptide: strict or relaxed

AMP-binding (508..895): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1551..1948): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNMSLIWK
Nearest Stachelhaus code(s):: Cys DLYNMSLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Atu3683

Search NORINE for peptide: strict or relaxed

AMP-binding (487..885): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLIWK
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1540..1927): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNCALTYK
Nearest Stachelhaus code(s):: Ala DLFNCALTYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)